Search results for " Genetically-Modified"

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Systematic gene overexpression in Candida albicans identifies a regulator of early adaptation to the mammalian gut.

2018

International audience; Candida albicans is part of the human gastrointestinal (GI) microbiota. To better understand how C. albicans efficiently establishes GI colonisation, we competitively challenged growth of 572 signature-tagged strains (~10% genome coverage), each conditionally overexpressing a single gene, in the murine gut. We identified CRZ2, a transcription factor whose overexpression and deletion respectively increased and decreased early GI colonisation. Using clues from genome-wide expression and gene-set enrichment analyses, we found that the optimal activity of Crz2p occurs under hypoxia at 37°C, as evidenced by both phenotypic and transcriptomic analyses following CRZ2 geneti…

0301 basic medicine[SDV.MHEP.AHA] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]030106 microbiologyImmunologyMicrobiologyMannosyltransferasesBiological pathwayTranscriptomeFungal ProteinsMannans03 medical and health scienceschemistry.chemical_compoundtranscriptomicsregulatory networksCell WallVirologyGene Expression Regulation FungalCandida albicanssignature‐tagged overexpression[SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]AnimalsGene Regulatory NetworksCandida albicansPromoter Regions GeneticGeneTranscription factorResearch ArticlesFungal proteinMice Inbred BALB CCRZ2chromatin immunoprecipitation‐on‐chipbiologyCRZ2;Candida albicans;chromatin immunoprecipitation-on-chip;gastrointestinal colonisation;regulatory networks;signature-tagged overexpression;transcriptomicsTunicamycinTunicamycinHydrogen-Ion Concentrationbiology.organism_classificationPhenotypeCell biologyGastrointestinal MicrobiomeGastrointestinal Tractchemistrychromatin immunoprecipitation-on-chipFemalesignature-tagged overexpressionMicroorganisms Genetically-Modifiedgastrointestinal colonisationResearch Article
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Evolution and functional differentiation of recently diverged phytochelatin synthase genes from Arundo donax L.

2019

Plant phytochelatin synthases undergo evolutionarily rapid functional differentiation after duplication, allowing fast and precise adjustment of metal detoxification capacity by modulation of both transcription and enzymatic activity.

Settore BIO/01 - BOTANICA GENERALE0106 biological sciences0301 basic medicineGene duplicationPhysiologyArabidopsisSaccharomyces cerevisiaePlant SciencePoaceae01 natural sciencesGenomeDivergenceEvolution Molecular03 medical and health sciencesGene Expression Regulation Plantcadmium; divergence; gene duplication; giant reed; phytochelatin synthase; phytochelatins; subfunctionalizationSubfunctionalizationPhytochelatinsArabidopsis thalianaAmino Acid SequenceGenePhylogenyPlant ProteinsGeneticsNatural selectionGiant reedbiologyArundo donaxAminoacyltransferasesPlants Genetically Modifiedbiology.organism_classificationResearch PapersPhenotype030104 developmental biologyPlant—Environment InteractionsSubfunctionalizationPhytochelatinMicroorganisms Genetically-ModifiedPhytochelatin synthaseSequence AlignmentCadmium010606 plant biology & botanyJournal of Experimental Botany
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